RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-RPH3AL Antibody

Catalog #: 144-04311

RPH3AL Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q9UNE2
Gene Id
9501
Gene Symbols
RPH3AL
Protein Name / Synonyms
RPH3AL, NOC2, rab effector Noc2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
31kDa/34kDa
Observed MW
45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 161-315 of human RPH3AL (NP_008918.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RPH3AL antibody (144-04311) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - RPH3AL antibody (144-04311)

Immunohistochemistry of paraffin-embedded human liver using RPH3AL antibody (144-04311) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPH3AL antibody (144-04311)

Immunohistochemistry of paraffin-embedded human stomach using RPH3AL antibody (144-04311) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPH3AL antibody (144-04311)

Expiration:
12 months from the date of shipment when stored properly.