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Anti-RPIA Antibody
Catalog #: 144-08867
RPIA Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49247 |
| Gene Id | 22934 |
| Gene Symbols | RPIA |
| Protein Name / Synonyms | RPIA, RPI, RPIAD, ribose-5-phosphate isomerase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18.
Dilutions
WB 1:200 - 1:1000
Format
Calculated MW
33kDa
Observed MW
33kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 82-311 of human RPIA (NP_653164.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RPIA antibody (144-08867) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.