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Anti-RPL8 Antibody

Catalog #: 144-10042

RPL8 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P62917
Gene Id
6132
Gene Symbols
RPL8
Protein Name / Synonyms
RPL8, L8, ribosomal protein L8
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Dilutions

WB 1:1000 - 1:4000
IF 1:100 - 1:200

Format

Calculated MW
28kDa
Observed MW
28kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human RPL8 (NP_000964.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RPL8 antibody (144-010042) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - RPL8 Polyclonal Antibody (144-010042)

Immunofluorescence analysis of HeLa cells using RPL8 antibody (144-010042) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - RPL8 Polyclonal Antibody (144-010042)

Expiration:
12 months from the date of shipment when stored properly.