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Anti-RPLP0 Antibody

Catalog #: 144-60420

RPLP0 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P05388
Gene Id
6175
Gene Symbols
RPLP0
Protein Name / Synonyms
RPLP0, L10E, LP0, P0, PRLP0, RPP0, 60S acidic ribosomal protein P0
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
27kDa/34kDa
Observed MW
37kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human RPLP0 (NP_000993.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RPLP0 antibody (144-013633) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - RPLP0 antibody (144-013633)

Immunohistochemistry of paraffin-embedded human prostate using RPLP0 Antibody (144-013633) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPLP0 Antibody (144-013633)

Immunohistochemistry of paraffin-embedded human esophagus using RPLP0 Antibody (144-013633) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPLP0 Antibody (144-013633)

Immunohistochemistry of paraffin-embedded mouse lung using RPLP0 Antibody (144-013633) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPLP0 Antibody (144-013633)

Immunofluorescence analysis of HeLa cells using RPLP0 antibody (144-013633). Blue: DAPI for nuclear staining.
Immunofluorescence - RPLP0 antibody (144-013633)

Expiration:
12 months from the date of shipment when stored properly.