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Anti-RPS12 Antibody
Catalog #: 144-05890
RPS12 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P25398 |
| Gene Id | 6206 |
| Gene Symbols | RPS12 |
| Protein Name / Synonyms | RPS12, S12, ribosomal protein S12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
14kDa
Observed MW
15kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human RPS12 (NP_001007.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RPS12 antibody (144-05890) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using RPS12 antibody (144-05890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using RPS12 antibody (144-05890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS12 antibody (144-05890) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RPS12 antibody (144-05890). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.