Anti-RPS13 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P62277
Gene Id	6207
Gene Symbols	RPS13
Protein Name / Synonyms	RPS13 (Center)
Clonality	Polyclonal
Preparation	This RPS13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the Central region of human RPS13.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

RPS13, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S15P family of ribosomal proteins. It is located in the cytoplasm. The protein has been shown to bind to the 5.8S rRNA in rat. The gene product of the E. coli ortholog (ribosomal protein S15) functions at early steps in ribosome assembly. This gene is co-transcribed with two U14 small nucleolar RNA genes, which are located in its third and fifth introns. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Chicken, Hamster, Xenopus

Other Names

RPS13; 40S ribosomal protein S13

NCBI Accession #

NP_001008.1

Other Accession #

P49393; P62278; P62301; Q9WVH0; Q6ITC7; Q56JX8

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

17222

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of RPS13 Antibody (Center) (Cat# 102-15766) in NCI-H460 cell line lysates (35ug/lane). RPS13 (arrow) was detected using the purified Pab.
	RPS13 Antibody (Center) (Cat# 102-15766) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS13 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	RPS13 Antibody (Center) (Cat# 102-15766) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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