RayBiotech

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Anti-RPS14 Antibody

Catalog #: 144-06727

RPS14 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P62263
Gene Id
6208
Gene Symbols
RPS14
Protein Name / Synonyms
RPS14, EMTB, S14, ribosomal protein S14
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
16kDa
Observed MW
16kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-151 of human RPS14 (NP_005608.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RPS14 antibody (144-06727) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - RPS14 Polyclonal Antibody (144-06727)

Western blot analysis of extracts of various cells, using RPS14 antibody (144-06727) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - RPS14 Polyclonal Antibody (144-06727)

Immunohistochemistry of paraffin-embedded mouse brain using RPS14 antibody (144-06727) at dilution of 1:100 (40x lens).
Immunohistochemistry - RPS14 Polyclonal Antibody (144-06727)

Expiration:
12 months from the date of shipment when stored properly.