RRM1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P23921 |
| Gene Id | 6240 |
| Gene Symbols | RRM1 |
| Protein Name / Synonyms | RRM1, R1, RIR1, RR1, ribonucleotide reductase catalytic subunit M1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes the large and catalytic subunit of ribonucleotide reductase, an enzyme essential for the conversion of ribonucleotides into deoxyribonucleotides. A pool of available deoxyribonucleotides is important for DNA replication during S phase of the cell cycle as well as multiple DNA repair processes. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:20 - 1:50
IF 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
90kDa
Observed MW
95kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 593-792 of human RRM1 (NP_001024.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RRM1 antibody (144-602928) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using RRM1 antibody (144-602928) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug RRM1 antibody (144-602928). Western blot was performed from the immunoprecipitate using RRM1 antibody (144-602928) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.
