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Anti-RRM2 Antibody
Catalog #: 144-05255
RRM2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P31350 |
| Gene Id | 6241 |
| Gene Symbols | RRM2 |
| Protein Name / Synonyms | RRM2, R2, RR2, RR2M, ribonucleotide reductase regulatory subunit M2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes one of two non-identical subunits for ribonucleotide reductase. This reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. Transcription from this gene can initiate from alternative promoters, which results in two isoforms that differ in the lengths of their N-termini. Related pseudogenes have been identified on chromosomes 1 and X.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:10 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:10 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
44kDa/51kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human RRM2 (NP_001025.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using RRM2 antibody (144-05255) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse lung using RRM2 antibody (144-05255) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using RRM2 antibody (144-05255) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.