Anti-RT25 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P82663
Gene Id	64432
Gene Symbols	MRPS25
Protein Name / Synonyms	RT25 (C-term)
Clonality	Polyclonal
Preparation	This RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the C-terminal region of human RT25.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.

Specificity

Other Names

MRPS25; RPMS25; 28S ribosomal protein S25, mitochondrial

NCBI Accession #

NP_071942.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

20116

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of RT25 Antibody (C-term) (Cat# 102-18417) in MCF-7 cell line lysates (35ug/lane). RT25 (arrow) was detected using the purified Pab.
	RT25 Antibody (C-term) (Cat# 102-18417) IHC analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RT25 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	RT25 Antibody (C-term) (Cat# 102-18417) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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