Anti-RUFY1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Mouse, Rat
Host Species	Rabbit
Accession Number	Q96T51
Gene Id	80230
Gene Symbols	RUFY1
Protein Name / Synonyms	RUFY1, RABIP4, ZFYVE12, RUN and FYVE domain containing 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein that contains a RUN domain and a FYVE-type zinc finger domain. The encoded protein binds to phosphatidylinositol-3-phosphate (PI3P) and plays a role in early endosomal trafficking, tethering and fusion through interactions with small GTPases including Rab4, Rab5 and Rab14. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

44kDa/69kDa/79kDa

Observed MW

80kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 439-708 of human RUFY1 (NP_079434.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RUFY1 antibody (144-07836) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Western blot - RUFY1 antibody (144-07836)

Immunohistochemistry of paraffin-embedded rat brain using RUFY1 antibody (144-07836) at dilution of 1:100 (40x lens).
Immunohistochemistry - RUFY1 antibody (144-07836)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products