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Anti-RUNX1 Antibody
Catalog #: 144-02055
RUNX1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q01196 |
| Gene Id | 861 |
| Gene Symbols | RUNX1 |
| Protein Name / Synonyms | RUNX1, AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha, runt-related transcription factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:50 - 1:200
Format
Calculated MW
20-28kDa/37kDa/48-51kDa
Observed MW
65kDa
Immunogen
A synthetic peptide of human RUNX1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of SH-SY5Y cells, using RUNX1 antibody (144-02055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat brain using RUNX1 Antibody (144-02055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using RUNX1 Antibody (144-02055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RUNX1 Antibody (144-02055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using RUNX1 Antibody (144-02055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using RUNX1 Antibody (144-02055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using RUNX1 Antibody (144-02055) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RUNX1 Antibody (144-02055) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using RUNX1 antibody (144-02055). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.