Anti-RUNX2 (S533) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13950
Gene Id	860
Gene Symbols	RUNX2,OSF2,AML3,CBFA1,PEBP2A
Protein Name / Synonyms	Runt-related transcription factor 2, Acutemyeloid leukemia 3 protein, Core-bindingfactor subunit alpha-1, CBF-alpha-1,Oncogene AML-3, Osteoblast-specific transcription factor 2, OSF-2, Polyomavirusenhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKVcore-binding factor alpha A subunit, RUNX2,AML3, CBFA1, OSF2, PEBP2A
Clonality	Polyclonal
Preparation	This RUNX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids surrounding S533 of human RUNX2.
Isotype	Kappa, Rabbit
Concentration (lot specific)	0.3 mg/ml
Buffers	PBS with 0.09% (W/V) sodium azide
Recommended Applications	Western Blotting, IHC-P, FC
Domain / Region / Terminus	445-474
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Runx2 is a member of the RUNX family of transcription factors. It is a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. It can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in the Runx2 gene have been associated with the bone development disorder cleidocranial dysplasia (CCD).

Specificity

NCBI Accession #

NP_001015051.3;NP_001019801.3

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

56648

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	RUNX2 Antibody (S533) (Cat# 102-17593) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RUNX2 antibody detected the RUNX2 protein (arrow).
	RUNX2 Antibody (S533) (102-17593)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX2 Antibody (S533) for immunohistochemistry. Clinical relevance has not been evaluated.
	RUNX2 Antibody (S533) (Cat# 102-17593) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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