RayBiotech

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Anti-RUVBL2 Antibody

Catalog #: 144-12564

RUVBL2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9Y230
Gene Id
10856
Gene Symbols
RUVBL2
Protein Name / Synonyms
RUVBL2, CGI-46, ECP-51, ECP51, INO80J, REPTIN, RVB2, TAP54-beta, TIH2, TIP48, TIP49B, ruvB-like 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
46kDa/51kDa
Observed MW
51kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human RUVBL2 (NP_006657.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using RUVBL2 antibody (144-12564) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - RUVBL2 antibody (144-12564)

Immunohistochemistry of paraffin-embedded rat lung using RUVBL2 antibody (144-12564) at dilution of 1:100 (40x lens).
Immunohistochemistry - RUVBL2 antibody (144-12564)

Immunohistochemistry of paraffin-embedded mouse brain using RUVBL2 antibody (144-12564) at dilution of 1:100 (40x lens).
Immunohistochemistry - RUVBL2 antibody (144-12564)

Immunofluorescence analysis of MCF-7 cells using RUVBL2 antibody (144-12564). Blue: DAPI for nuclear staining.
Immunofluorescence - RUVBL2 antibody (144-12564)

Expiration:
12 months from the date of shipment when stored properly.