Anti-S100A4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P26447
Gene Id	6275
Gene Symbols	S100A4
Protein Name / Synonyms	S100A4, 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, protein S100-A4
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

11kDa

Observed MW

12kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A4 (NP_002952.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using S100A4 antibody (144-01631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Western blot analysis of extracts from normal (control) and S100A4 knockout (KO) HeLa cells, using S100A4 antibody (144-01631) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
Western blot - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Immunohistochemistry of paraffin-embedded rat brain using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Immunohistochemistry of paraffin-embedded human mammary gland using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Immunohistochemistry of paraffin-embedded human gastric cancer using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Immunohistochemistry of paraffin-embedded mouse lung using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Immunohistochemistry of paraffin-embedded human tonsil using S100A4 antibody (144-01631) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Immunofluorescence analysis of HeLa cells using S100A4 antibody (144-01631) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Immunofluorescence analysis of PC12 cells using S100A4 antibody (144-01631) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Immunofluorescence analysis of RAW264.7 cells using S100A4 antibody (144-01631) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] S100A4 Polyclonal Antibody (144-01631)

Expiration:

12 months from the date of shipment when stored properly.

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