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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15424 |
| Gene Id | 6294 |
| Gene Symbols | SAFB |
| Protein Name / Synonyms | SAFB, HAP, HET, SAB-B1, SAF-B, SAF-B1, SAFB1, scaffold attachment factor B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
95kDa/102kDa
Observed MW
130kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SAFB (NP_001188267.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SAFB antibody (144-07927) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat lung using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SAFB antibody (144-07927) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.