Anti-SAMHD1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9Y3Z3
Gene Id	25939
Gene Symbols	SAMHD1
Protein Name / Synonyms	SAMHD1, CHBL2, DCIP, HDDC1, MOP-5, SBBI88, deoxynucleoside triphosphate triphosphohydrolase SAMHD1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

63kDa/68kDa/72kDa

Observed MW

72kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 387-626 of human SAMHD1 (NP_056289.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SAMHD1 antibody (144-07794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] SAMHD1 Polyclonal Antibody (144-07794)

Western blot analysis of extracts from normal (control) and SAMHD1 knockout (KO) HeLa cells, using SAMHD1 antibody (144-07794) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.
Western blot - [KO Validated] SAMHD1 Polyclonal Antibody (144-07794)

Immunofluorescence analysis of A549 cells using SAMHD1 antibody (144-07794). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] SAMHD1 Polyclonal Antibody (144-07794)

Expiration:

12 months from the date of shipment when stored properly.

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