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Anti-SARDH Antibody
Catalog #: 144-09541
SARDH Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9UL12 |
| Gene Id | 1757 |
| Gene Symbols | SARDH |
| Protein Name / Synonyms | SARDH, BPR-2, DMGDHL1, SAR, SARD, SDH, sarcosine dehydrogenase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an enzyme localized to the mitochondrial matrix which catalyzes the oxidative demethylation of sarcosine. This enzyme is distinct from another mitochondrial matrix enzyme, dimethylglycine dehydrogenase, which catalyzes a reaction resulting in the formation of sarcosine. Mutations in this gene are associated with sarcosinemia. Alternatively spliced transcript variants have been described.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
82kDa/101kDa
Observed MW
105kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SARDH Antibody (144-09541) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.