RayBiotech

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ISO 13485:2016 cGMP

Anti-SBDS Antibody

Catalog #: 144-05876

SBDS Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9Y3A5
Gene Id
51119
Gene Symbols
SBDS
Protein Name / Synonyms
SBDS, CGI-97, SDS, SWDS, ribosome maturation protein SBDS
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of a highly conserved protein family that exists from archaea to vertebrates and plants. The encoded protein may function in RNA metabolism. Mutations within this gene are associated with Shwachman-Bodian-Diamond syndrome. An alternative transcript has been described, but its biological nature has not been determined. This gene has a closely linked pseudogene that is distally located.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200

Format

Calculated MW
28kDa
Observed MW
34kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SBDS (NP_057122.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SBDS antibody (144-05876) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - SBDS antibody (144-05876)

Immunohistochemistry of paraffin-embedded human gastric cancer using SBDS antibody (144-05876) at dilution of 1:100 (40x lens).
Immunohistochemistry - SBDS antibody (144-05876)

Immunofluorescence analysis of MCF-7 cells using SBDS antibody (144-05876). Blue: DAPI for nuclear staining.
Immunofluorescence - SBDS antibody (144-05876)

Expiration:
12 months from the date of shipment when stored properly.