RayBiotech

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ISO 13485:2016 cGMP

Anti-SBF1 Antibody

Catalog #: 144-12354

SBF1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Rat
Host SpeciesRabbit
Accession Number
O95248
Gene Id
6305
Gene Symbols
SBF1
Protein Name / Synonyms
SBF1, CMT4B3, DENND7A, MTMR5, SET binding factor 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the protein-tyrosine phosphatase family. However, the encoded protein does not appear to be a catalytically active phosphatase because it lacks several amino acids in the catalytic pocket. This protein contains a Guanine nucleotide exchange factor (GEF) domain which is necessary for its role in growth and differentiation. Mutations in this gene have been associated with Charcot-Marie-Tooth disease 4B3. Pseudogenes of this gene have been defined on chromosomes 1 and 8.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
208kDa/211kDa
Observed MW
208kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1770 of human SBF1 (NP_002963.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SBF1 antibody (144-12354) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - SBF1 antibody (144-12354)

Expiration:
12 months from the date of shipment when stored properly.