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Anti-SCG2 Antibody
Catalog #: 144-06425
SCG2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P13521 |
| Gene Id | 7857 |
| Gene Symbols | SCG2 |
| Protein Name / Synonyms | SCG2, CHGC, EM66, SN, SgII, secretogranin-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
70kDa
Observed MW
65kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-320 of human SCG2 (NP_003460.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SCG2 antibody (144-06425) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using SCG2 antibody (144-06425). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.