Anti-SEC61A1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	29927
Protein Name / Synonyms	SEC61A1
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

HSEC61 antibody, SEC61 antibody, SEC61A antibody, SEC61A1 antibody, protein transport protein SEC61 alpha subunit antibody, sec61 homolog antibody, Sec61 alpha-1 antibody, protein transport protein Sec61 subunit alpha antibody, protein transport protein Sec61 subunit alpha isoform 1 antibody, Sec61 alpha 1 subunit (S. cerevisiae) antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

52 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 413 and 476 of SEC61A1

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using SEC61A1(107-10625) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
107-10625 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SEC61A1(107-10625) antibody at 1:500 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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