3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-SELE Antibody
Catalog #: 144-02191
SELE Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P16581 |
| Gene Id | 6401 |
| Gene Symbols | SELE |
| Protein Name / Synonyms | SELE, CD62E, ELAM, ELAM1, ESEL, LECAM2, E-selectin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
66kDa
Observed MW
68kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-230 of human SELE (NP_000441.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SELE antibody (144-02191) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast using SELE Antibody (144-02191) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using SELE Antibody (144-02191) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using SELE Antibody (144-02191) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using SELE antibody (144-02191). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.