Anti-SELENBP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q13228
Gene Id	8991
Gene Symbols	SELENBP1
Protein Name / Synonyms	SELENBP1, HEL-S-134P, LPSB, SBP56, SP56, hSBP, selenium-binding protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the selenium-binding protein family. Selenium is an essential nutrient that exhibits potent anticarcinogenic properties, and deficiency of selenium may cause certain neurologic diseases. The effects of selenium in preventing cancer and neurologic diseases may be mediated by selenium-binding proteins, and decreased expression of this gene may be associated with several types of cancer. The encoded protein may play a selenium-dependent role in ubiquitination/deubiquitination-mediated protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

45kDa/52kDa/56kDa

Observed MW

55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 243-472 of human SELENBP1 (NP_003935.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SELENBP1 antibody (144-01222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - [KO Validated] SELENBP1 Polyclonal Antibody (144-01222)

Western blot analysis of extracts from normal (control) and SELENBP1 knockout (KO) HeLa cells, using SELENBP1 antibody (144-01222) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - [KO Validated] SELENBP1 Polyclonal Antibody (144-01222)

Immunohistochemistry of paraffin-embedded human colon using SELENBP1 Antibody (144-01222) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SELENBP1 Polyclonal Antibody (144-01222)

Immunofluorescence analysis of HeLa cells using SELENBP1 antibody (144-01222). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] SELENBP1 Polyclonal Antibody (144-01222)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products