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Anti-SELP Antibody
Catalog #: 144-01425
SELP Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P16109 |
| Gene Id | 6403 |
| Gene Symbols | SELP |
| Protein Name / Synonyms | SELP, CD62, CD62P, GMP140, GRMP, LECAM3, PADGEM, PSEL, P-selectin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
90kDa
Observed MW
115kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human SELP (NP_002996.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SELP antibody (144-01425) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human prostate using SELP antibody (144-01425) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using SELP antibody (144-01425) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SELP antibody (144-01425) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.