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Anti-SEPSECS Antibody
Catalog #: 144-07103
SEPSECS Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9HD40 |
| Gene Id | 51091 |
| Gene Symbols | SEPSECS |
| Protein Name / Synonyms | SEPSECS, LP, PCH2D, SLA, SLA/LP, O-phosphoseryl-tRNA(Sec) selenium transferase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
5kDa/55kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human SEPSECS (NP_058651.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SEPSECS Antibody (144-07103) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Immunofluorescence analysis of U2OS cells using SEPSECS antibody (144-07103). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.