Anti-SERPINA3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P01011
Gene Id	12
Gene Symbols	SERPINA3
Protein Name / Synonyms	SERPINA3, AACT, ACT, GIG24, GIG25, alpha-1-antichymotrypsin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200

Format

Calculated MW

10kDa/24kDa/47kDa

Observed MW

50kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-423 of human SERPINA3 (NP_001076.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SERPINA3 antibody (144-01021) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - SERPINA3 Polyclonal Antibody (144-01021)

Immunohistochemistry of paraffin-embedded rat brain using SERPINA3 antibody (144-01021) at dilution of 1:100 (40x lens).
Immunohistochemistry - SERPINA3 Polyclonal Antibody (144-01021)

Immunohistochemistry of paraffin-embedded mouse lung using SERPINA3 antibody (144-01021) at dilution of 1:100 (40x lens).
Immunohistochemistry - SERPINA3 Polyclonal Antibody (144-01021)

Immunofluorescence analysis of A549 cells using SERPINA3 antibody (144-01021). Blue: DAPI for nuclear staining.
Immunofluorescence - SERPINA3 Polyclonal Antibody (144-01021)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products