SF3B2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13435 |
| Gene Id | 10992 |
| Gene Symbols | SF3B2 |
| Protein Name / Synonyms | SF3B2, Cus1, SAP145, SF3B145, SF3b1, SF3b150, splicing factor 3B subunit 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
100kDa
Observed MW
140kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 616-895 of human SF3B2 (NP_006833.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SF3B2 antibody (144-05875) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat liver using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse Intestine using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using SF3B2 antibody (144-05875) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using SF3B2 antibody (144-05875).
Expiration:
12 months from the date of shipment when stored properly.