RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-SFN Antibody

Catalog #: 144-01026

SFN Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P31947
Gene Id
2810
Gene Symbols
SFN
Protein Name / Synonyms
SFN, YWHAS, 14-3-3 protein sigma, stratifin
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW
24kDa/27kDa
Observed MW
31kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human SFN (NP_006133.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SFN antibody (144-01026) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded rat lung using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded rat liver using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded rat brain using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded rat kidney using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded human liver injury using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded human kidney using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded human esophagus using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded mouse liver using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded mouse kidney using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded rat lung using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded rat liver using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded rat spleen using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded rat kidney using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded human liver using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded mouse liver using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunohistochemistry of paraffin-embedded mouse kidney using SFN Antibody (144-01026) at dilution of 1:100 (40x lens).
Immunohistochemistry - SFN Polyclonal Antibody (144-01026)

Immunofluorescence analysis of MCF-7 cells using SFN antibody (144-01026). Blue: DAPI for nuclear staining.
Immunofluorescence - SFN Polyclonal Antibody (144-01026)

Expiration:
12 months from the date of shipment when stored properly.