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Anti-SFPQ Antibody
Catalog #: 144-00958
SFPQ Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P23246 |
| Gene Id | 6421 |
| Gene Symbols | SFPQ |
| Protein Name / Synonyms | SFPQ, POMP100, PPP1R140, PSF, splicing factor proline and glutamine rich |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
72kDa/76kDa
Observed MW
115kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 578-707 of human SFPQ (NP_005057.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SFPQ antibody (144-00958) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot analysis of extracts of various cells, using SFPQ antibody (144-00958) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:4000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using SFPQ antibody (144-00958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SFPQ antibody (144-00958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using SFPQ antibody (144-00958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SFPQ antibody (144-00958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using SFPQ antibody (144-00958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SFPQ antibody (144-00958) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using SFPQ antibody (144-00958) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.