Anti-SGK1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Mouse, Rat
Host Species	Rabbit
Accession Number	O00141
Gene Id	6446
Gene Symbols	SGK1
Protein Name / Synonyms	SGK1, SGK, serine/threonine-protein kinase Sgk1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

47kDa/48kDa/50kDa/52kDa/59kDa

Observed MW

60kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 172-431 of human SGK1 (NP_005618.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SGK1 antibody (144-01025) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Western blot - SGK1 Polyclonal Antibody (144-01025)

Western blot analysis of extracts of various cell lines, using SGK1 antibody (144-01025) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 300s.
Western blot - SGK1 Polyclonal Antibody (144-01025)

Immunohistochemistry of paraffin-embedded human esophagus using SGK1 antibody (144-01025) at dilution of 1:100 (40x lens).
Immunohistochemistry - SGK1 Polyclonal Antibody (144-01025)

Immunofluorescence analysis of HeLa cells using SGK1 antibody (144-01025). Blue: DAPI for nuclear staining.
Immunofluorescence - SGK1 Polyclonal Antibody (144-01025)

Expiration:

12 months from the date of shipment when stored properly.

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