Anti-SGSH Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P51688
Gene Id	6448
Gene Symbols	SGSH
Protein Name / Synonyms	SGSH, HSS, MPS3A, SFMD, N-sulfoglucosamine sulfohydrolase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

56kDa

Observed MW

57kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 273-502 of human SGSH (NP_000190.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SGSH antibody (144-08148) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - SGSH antibody (144-08148)

Immunohistochemistry of paraffin-embedded human liver using SGSH antibody (144-08148) at dilution of 1:100 (40x lens).
Immunohistochemistry - SGSH antibody (144-08148)

Immunohistochemistry of paraffin-embedded human stomach using SGSH antibody (144-08148) at dilution of 1:100 (40x lens).
Immunohistochemistry - SGSH antibody (144-08148)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products