Anti-SGTA Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O43765
Gene Id	6449
Gene Symbols	SGTA
Protein Name / Synonyms	SGTA, SGT, alphaSGT, hSGT, small glutamine rich tetratricopeptide repeat containing alpha
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

34kDa

Observed MW

38kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SGTA antibody (144-07306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] SGTA Polyclonal Antibody (144-07306)

Western blot analysis of extracts from normal (control) and SGTA knockout (KO) 293T cells, using SGTA antibody (144-07306) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] SGTA Polyclonal Antibody (144-07306)

Immunohistochemistry of paraffin-embedded human liver cancer using SGTA antibody (144-07306) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SGTA Polyclonal Antibody (144-07306)

Immunofluorescence analysis of A-549 cells using SGTA antibody (144-07306). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] SGTA Polyclonal Antibody (144-07306)

Expiration:

12 months from the date of shipment when stored properly.

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