SHC1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P29353 |
| Gene Id | 6464 |
| Gene Symbols | SHC1 |
| Protein Name / Synonyms | SHC1, SHC, SHCA, SHC adaptor protein 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
40kDa/46kDa/51kDa/62kDa
Observed MW
46kDa, 52kDa, 66kDa
Immunogen
A synthetic peptide of human SHC1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SHC1 antibody (144-07725) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using SHC1 antibody (144-07725) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney using SHC1 antibody (144-07725) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SHC1 antibody (144-07725) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SHC1 antibody (144-07725) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.