3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-SHMT2 Antibody
Catalog #: 144-01215
SHMT2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P34897 |
| Gene Id | 6472 |
| Gene Symbols | SHMT2 |
| Protein Name / Synonyms | SHMT2, GLYA, HEL-S-51e, SHMT, serine hydroxymethyltransferase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
53kDa/54kDa/55kDa
Observed MW
52kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SHMT2 antibody (144-01215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat kidney using SHMT2 Antibody (144-01215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SHMT2 Antibody (144-01215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SHMT2 Antibody (144-01215) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SHMT2 antibody (144-01215). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.