Anti-SHP2 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q06124
Gene Id	5781
Gene Symbols	PTPN11
Protein Name / Synonyms	SHP2
Clonality	Polyclonal
Preparation	This SHP2 antibody is generated from rabbits immunized with a recombinant protein encoding human SHP2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

SHP2, also known as PTPN11, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in the gene are a cause of Noonan syndrome as well as acute myeloid leukemia.

Specificity

Other Names

PTPN11; PTP2C; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; SH-PTP2; SH-PTP3

NCBI Accession #

NP_002825.3;NP_542168.1

Other Accession #

NP_002825

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

68436

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of Hela cell stained with SHP2 Antibody(Cat# 102-17887/SH050329B).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SHP2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).SHP2 immunoreactivity is localized to Nucleolus and Cytoplasm significantly and Nucleus weakly.

Western blot analysis of PTPN11 (arrow) using rabbit polyclonal SHP2 Antibody (RB07283). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTPN11 gene (Lane 2) (Origene Technologies).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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