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Anti-SIAH1 Antibody
Catalog #: 144-02494
SIAH1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8IUQ4 |
| Gene Id | 6477 |
| Gene Symbols | SIAH1 |
| Protein Name / Synonyms | SIAH1, SIAH1A, E3 ubiquitin-protein ligase SIAH1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
21kDa/31kDa/34kDa
Observed MW
31kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SIAH1 (NP_001006611.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse kidney, using SIAH1 antibody (144-02494) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using SIAH1 antibody (144-02494) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using SIAH1 antibody (144-02494) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using SIAH1 antibody (144-02494) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SIAH1 antibody (144-02494) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SIAH1 antibody (144-02494) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using SIAH1 antibody (144-02494) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SIAH1 antibody (144-02494). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.