Anti-SIAT4A Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	6482
Protein Name / Synonyms	SIAT4A
Concentration (lot specific)	0.98 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DKFZp666E036 antibody, DKFZp779K2051 antibody, FLJ36548 antibody, Gal-NAc6S antibody, MGC9183 antibody, SIAT4A antibody, SIATFL antibody, ST3GalA antibody, ST3GalA.1 antibody, ST3GalIA antibody, "ST3GalIA,1 antibody", ST3O antibody, ST3GAL1 antibody, "sialyltransferase 4A (beta-galactosidase alpha-2,3-sialytransferase) antibody", "sialyltransferase 4A (beta-galactoside alpha-2,3-sialyltransferase) antibody", "beta-galactoside alpha-2,3-sialyltransferase 1 antibody", SIAT4-A antibody, "Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase antibody", "sialyltransferase 4A (beta-galactoside alpha-2,3-sialytransferase) antibody", "CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1 antibody", "alpha 2,3-ST 1 antibody", ST3GalI antibody, "ST3 beta-galactoside alpha-2,3-sialyltransferase 1 antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

39 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 195 of SIAT4A (Uniprot ID# Q11201)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed Hep3B, using SIAT4A(107-10584) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded serous OVCA, using ST3GAL1(107-10584) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A:HeLa S3
12% SDS PAGE
107-10584 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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