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Anti-SIM1 (N-term) Antibody
Catalog #: 102-11807
Rabbit Anti-Human SIM1 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P81133 |
| Gene Id | 6492 |
| Gene Symbols | SIM1 |
| Protein Name / Synonyms | SIM1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This SIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIM1. |
| Concentration (lot specific) | 0.500 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome.
Specificity
Predicted Reactivity
Mouse, Drosophila
Other Names
SIM1; BHLHE14; Single-minded homolog 1; Class E basic helix-loop-helix protein 14
NCBI Accession #
NP_005059.2
Other Accession #
P05709; Q61045; NP_005059.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
85515
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of lysate from 293T cell line, using SIM1 Antibody (N-term) (Cat# 102-11807). 102-11807 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. |
 | SIM1 Antibody (N-term) (Cat# 102-11807)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIM1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.