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Anti-SIN3A Antibody
Catalog #: 144-01577
SIN3A Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q96ST3 |
| Gene Id | 25942 |
| Gene Symbols | SIN3A |
| Protein Name / Synonyms | SIN3A, WITKOS, paired amphipathic helix protein Sin3a |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP, ChIP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
ChIP 1:20 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:100
ChIP 1:20 - 1:100
Format
Calculated MW
145kDa
Observed MW
165kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SIN3A (NP_056292.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SIN3A antibody (144-01577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using SIN3A antibody (144-01577). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SIN3A antibody (144-01577). Western blot was performed from the immunoprecipitate using SIN3A antibody (144-01577) at a dilition of 1:500.
Expiration:
12 months from the date of shipment when stored properly.