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Anti-SIRT3 Antibody
Catalog #: 144-07307
SIRT3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NTG7 |
| Gene Id | 23410 |
| Gene Symbols | SIRT3 |
| Protein Name / Synonyms | SIRT3, SIR2L3, sirtuin 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.01% thimerosal, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:100 - 1:200
IHC 1:50 - 1:200
IF 1:100 - 1:200
Format
Calculated MW
28kDa/43kDa
Observed MW
44kDa, 27kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SIRT3 (NP_036371.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SIRT3 antibody (144-07307) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 antibody (144-07307) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using SIRT3 antibody (144-07307). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.