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Anti-SLC12A1 Antibody
Catalog #: 144-60140
SLC12A1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13621 |
| Gene Id | 6557 |
| Gene Symbols | SLC12A1 |
| Protein Name / Synonyms | SLC12A1, BSC1, NKCC2, solute carrier family 12 member 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a kidney-specific sodium-potassium-chloride cotransporter that is expressed on the luminal membrane of renal epithelial cells of the thick ascending limb of Henle's loop and the macula densa. It plays a key role in concentrating urine and accounts for most of the NaCl resorption. It is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity in humans has not been experimentally proven.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
121kDa
Observed MW
150kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SLC12A1 (NP_000329.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SLC12A1 antibody (144-60140) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.