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Anti-SLC1A2 Antibody
Catalog #: 144-00910
SLC1A2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P43004 |
| Gene Id | 6506 |
| Gene Symbols | SLC1A2 |
| Protein Name / Synonyms | SLC1A2, EAAT2, EIEE41, GLT-1, HBGT, solute carrier family 1 member 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified.
Dilutions
WB 1:500 - 1:5000
IHC 1:100 - 1:200
IHC 1:100 - 1:200
Format
Calculated MW
61kDa/62kDa
Observed MW
65kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 495-574 of human SLC1A2 (NP_004162.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SLC1A2 antibody (144-00910) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using SLC1A2 antibody (144-00910) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using SLC1A2 antibody (144-00910) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SLC1A2 antibody (144-00910) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using SLC1A2 antibody (144-00910) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.