Anti-SLC25A5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat, Non-Human Primate (rhesus monkey)
Host Species	Rabbit
Accession Number	P05141
Gene Id	292
Gene Symbols	SLC25A5
Protein Name / Synonyms	SLC25A5, 2F1, AAC2, ANT2, T2, T3, ADP/ATP translocase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Suppressed expression of this gene has been shown to induce apoptosis and inhibit tumor growth. The human genome contains several non-transcribed pseudogenes of this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

32kDa

Observed MW

33kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SLC25A5 (NP_001143.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SLC25A5 antibody (144-015639) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - SLC25A5 Polyclonal Antibody (144-015639)

Immunofluorescence analysis of HeLa cells using SLC25A5 antibody (144-015639) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - SLC25A5 Polyclonal Antibody (144-015639)

Expiration:

12 months from the date of shipment when stored properly.

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