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Anti-SLC29A1 Antibody

Catalog #: 144-60238

SLC29A1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesMouse, Rat
Host SpeciesRabbit
Accession Number
Q99808
Gene Id
2030
Gene Symbols
SLC29A1
Protein Name / Synonyms
SLC29A1, ENT1, equilibrative nucleoside transporter 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
50kDa/58kDa
Observed MW
55kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human SLC29A1 (NP_001071645.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of rat lung, using SLC29A1 antibody (144-60238) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - SLC29A1 Polyclonal Antibody (144-60238)

Expiration:
12 months from the date of shipment when stored properly.