SLC2A4 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P14672 |
| Gene Id | 6517 |
| Gene Symbols | SLC2A4 |
| Protein Name / Synonyms | SLC2A4, GLUT4, solute carrier family 2 member 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
43kDa/54kDa
Observed MW
55kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human SLC2A4 (NP_001033.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SLC2A4 antibody (144-07637) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using SLC2A4 antibody (144-07637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SLC2A4 antibody (144-07637) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SLC2A4 antibody (144-07637) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.