Anti-SMARCA4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P51532
Gene Id	6597
Gene Symbols	SMARCA4
Protein Name / Synonyms	SMARCA4, BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b, transcription activator BRG1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:20 - 1:50

Format

Calculated MW

181kDa/184kDa

Observed MW

180kDa

Immunogen

A synthetic peptide of human SMARCA4

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SMARCA4 antibody (144-00887) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)

Western blot analysis of extracts from normal (control) and SMARCA4 knockout (KO) 293T cells, using SMARCA4 antibody (144-00887) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)

Immunohistochemistry of paraffin-embedded human colon using SMARCA4 antibody (144-00887) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)

Immunohistochemistry of paraffin-embedded human colon carcinoma using SMARCA4 antibody (144-00887) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)

Immunofluorescence analysis of HeLa cells using SMARCA4 antibody (144-00887).
Immunofluorescence - [KO Validated] SMARCA4 Polyclonal Antibody (144-00887)

Expiration:

12 months from the date of shipment when stored properly.

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