Anti-SMARCA5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O60264
Gene Id	8467
Gene Symbols	SMARCA5
Protein Name / Synonyms	SMARCA5, ISWI, SNF2H, WCRF135, hISWI, hSNF2H, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

121kDa

Observed MW

125kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-400 of human SMARCA5 (NP_003592.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SMARCA5 antibody (144-02000) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 2min.
Western blot - SMARCA5 antibody (144-02000)

Immunofluorescence analysis of HeLa cells using SMARCA5 antibody (144-02000). Blue: DAPI for nuclear staining.
Immunofluorescence - SMARCA5 antibody (144-02000)

Expiration:

12 months from the date of shipment when stored properly.

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