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Anti-SMARCB1 Antibody
Catalog #: 144-05767
SMARCB1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q12824 |
| Gene Id | 6598 |
| Gene Symbols | SMARCB1 |
| Protein Name / Synonyms | SMARCB1, BAF47, CSS3, INI1, MRD15, PPP1R144, RDT, RTPS1, SNF5, SNF5L1, SWNTS1, Sfh1p, Snr1, hSNFS, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
43kDa/44kDa
Observed MW
44kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 77-376 of human SMARCB1 (NP_001007469.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using SMARCB1 antibody (144-05767) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat Intestine using SMARCB1 antibody (144-05767) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using SMARCB1 antibody (144-05767) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SMARCB1 antibody (144-05767) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse osteosarcoma using SMARCB1 antibody (144-05767) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.