Anti-SMARCC1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q92922
Gene Id	6599
Gene Symbols	SMARCC1
Protein Name / Synonyms	SMARCC1, BAF155, CRACC1, Rsc8, SRG3, SWI3, SWI/SNF complex subunit SMARCC1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200

Format

Calculated MW

122kDa

Observed MW

155kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 80-320 of human SMARCC1 (NP_003065.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using SMARCC1 antibody (144-06128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.
Western blot - [KO Validated] SMARCC1 Polyclonal Antibody (144-06128)

Western blot analysis of extracts from normal (control) and SMARCC1 knockout (KO) HeLa cells, using SMARCC1 antibody (144-06128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
Western blot - [KO Validated] SMARCC1 Polyclonal Antibody (144-06128)

Immunohistochemistry of paraffin-embedded human kidney cancer using SMARCC1 antibody (144-06128) at dilution of 1:200 (40x lens).
Immunohistochemistry - [KO Validated] SMARCC1 Polyclonal Antibody (144-06128)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug SMARCC1 antibody (144-06128). Western blot was performed from the immunoprecipitate using SMARCC1 antibody (144-06128) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] SMARCC1 Polyclonal Antibody (144-06128)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products